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Single or Double-Lumen Aspiration Needle? Revisiting Choices for In Vitro Fertilization or Intracytoplasmic Sperm
Gopinath Muruti1, Mohd Faizal Ahmad1,2, Muhammad Azrai Abu1,2
1Advanced Reproductive Centre, Hospital Canselor Tuanku Muhriz, National University of Malaysia, Kuala Lumpur 56000, Malaysia.
Life (Basel, Switzerland)
|September 27, 2025
Summary
Single-lumen needles (SLN) and double-lumen needles (DLN) are effective for oocyte retrieval in assisted reproductive technology (ART). Studies show SLN offers shorter procedure times without compromising oocyte yield or pregnancy outcomes.
Area of Science:
- Reproductive Medicine
- Ultrasound-Guided Procedures
- Assisted Reproductive Technology (ART)
Background:
- Transvaginal ultrasound-guided follicle aspiration is the standard for oocyte retrieval in ART.
- Both single-lumen aspiration needles (SLN) and double-lumen aspiration needles (DLN) are utilized for this procedure.
Purpose of the Study:
- To systematically compare the clinical outcomes and impact of using SLN versus DLN in women undergoing ART.
- To evaluate differences in oocyte yield, procedural efficiency, and safety between SLN and DLN.
Main Methods:
- A systematic literature search was performed on PubMed and Google Scholar until May 2025.
- Five studies were included, comparing SLN and DLN in ART procedures.
- Outcomes analyzed included oocyte yield, MII oocytes, procedural duration, complications, fertilization rates, embryo quality, and pregnancy rates.
Main Results:
- No significant differences were found in oocyte yield or MII oocyte count between SLN and DLN.
- Procedures using SLN were significantly shorter than those using DLN.
- Fertilization rates, embryo quality, and clinical pregnancy, ongoing pregnancy, and live birth rates were comparable between the two needle types.
Conclusions:
- Single-lumen needles (SLN) demonstrate comparable efficacy to double-lumen needles (DLN) for oocyte retrieval in ART.
- SLN offers a potential advantage in reducing procedural duration without negatively impacting key ART outcomes.
- The choice between SLN and DLN may be based on procedural efficiency and patient factors.

