Anti-Inflammatory and Antioxidant Effects of Topical Formulations Containing Plant Extracts, Methylsulfonylmethane,
Thi Xoan Hoang1,2, Nhat Minh Dang1, Kang Gyu Bae3
1Department of Life Science, Gachon University, Seongnam 13120, Republic of Korea.
Abstract:
Background: This study aimed to evaluate the anti-inflammatory and antioxidant effects of AS632 and AS633, two topical formulations composed of natural plant-derived ingredients, for potential use in arthritis therapy. Methods: AS632 and AS633 were formulated with natural plant extracts-including Punica granatum seed oil, Gaultheria procumbens essential oil, Centella asiatica extract, and Camellia sinensis extract-and methylsulfonylmethane (MSM). AS632 additionally contained a peptide-based component, Peptiskin®. Both formulations were tested in THP-1-derived macrophages, HaCaT keratinocytes, and C28/I2 chondrocytes stimulated with lipopolysaccharide (LPS) or pro-inflammatory cytokines. Results: Both formulations significantly reduced TNF-α, IL-1β, matrix metalloproteinase (MMP-2, MMP-9, MMP-13) expression, and ROS level, with AS632 showing greater suppression of TNF-α in macrophages compared to AS633. In addition, both formulations demonstrated cytoprotective effects against cytokine-induced damage in chondrocytes. Conclusions: AS632 and AS633 are promising topical candidates for managing arthritis and chronic inflammatory skin or joint disorders.
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