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Experimental Infection in Mice with Cryptosporidium Isolated from Humans.

Rodica Georgiana Dărăbuș1,2, Marius Stelian Ilie3, Gheorghe Dărăbuș3

  • 1Discipline of Parasitology, Department of Infectious Diseases, Victor Babes University of Medicine and Pharmacy, 300041 Timisoara, Romania.

Pathogens (Basel, Switzerland)
|September 27, 2025
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Summary

Mice infected with three human Cryptosporidium species shed oocysts, indicating they can transmit the parasite. This study highlights mice as potential reservoirs for Cryptosporidium transmission to other species, including humans.

Keywords:
C. parvumC. ryanaeCryptosporidium canisexperimental infectionmice

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Area of Science:

  • Veterinary Parasitology
  • Infectious Diseases
  • Microbiology

Background:

  • Cryptosporidium is a protozoan parasite affecting the gastrointestinal and respiratory tracts of various hosts.
  • Human-derived Cryptosporidium species pose a significant public health concern.
  • Understanding host-parasite interactions is crucial for controlling transmission.

Purpose of the Study:

  • To experimentally infect conventional mice with three human-derived Cryptosporidium species.
  • To assess the infectivity and shedding patterns of Cryptosporidium canis, Cryptosporidium parvum, and Cryptosporidium ryanae in mice.
  • To evaluate the potential of mice as a reservoir for Cryptosporidium transmission.

Main Methods:

  • Oral inoculation of 10-day-old mice with C. canis, C. parvum, or C. ryanae.
  • Fecal sample analysis for oocyst detection starting four days post-infection.
  • Detection of infection using rapid immunochromatographic tests up to 40 days post-infection.
  • Monitoring of dam mice for indirect transmission.

Main Results:

  • Successful infection was achieved with all three Cryptosporidium species, albeit at low levels.
  • Oocysts were detected in feces from four days post-infection.
  • Infection was confirmed via immunochromatographic tests at 40 days post-infection.
  • Dam mice became infected by day 17, suggesting indirect transmission.

Conclusions:

  • Mice can be experimentally infected with human-derived Cryptosporidium species.
  • Mice may serve as a potential source for Cryptosporidium transmission to susceptible hosts, including humans.
  • Further research is needed to elucidate the role of rodents in Cryptosporidium epidemiology.