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Characterization and Role of AP2/EREBP Genes with Decreasing Expression During Leaf Development in 84K Poplar
Sanjiao Wang1, Nan Liu1, Jingna Si1
1State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
Researchers identified 76 AP2/EREBP genes in 84K poplar, revealing their roles in leaf development. Five specific genes showed decreasing expression during leaf development, offering insights into plant growth regulation.
Area of Science:
- Plant Biology
- Genomics
- Molecular Genetics
Background:
- The AP2/EREBP transcription factor family is crucial for plant growth, development, and stress responses.
- Understanding leaf development mechanisms is vital for improving plant traits.
Purpose of the Study:
- To investigate the roles of AP2/EREBP genes in 84K poplar leaf development.
- To analyze the evolutionary relationships and expression patterns of AP2/EREBP genes in poplar.
Main Methods:
- Bulk RNA sequencing and transcriptome analysis were used to identify AP2/EREBP genes.
- Phylogenetic analysis and structural domain characteristics classified the genes into subfamilies.
- RT-PCR and subcellular localization experiments validated gene expression and function.
Main Results:
- 76 AP2/EREBP genes were identified and classified into RAV, ERF, and AP2 subfamilies.
- Promoter analysis revealed a prevalence of light-responsive elements.
- Five genes (Pop_G10G022861, Pop_A01G003858, Pop_A01G081120, Pop_A01G074798, Pop_A07G010900) showed decreasing expression during leaf development.
- Two genes, Pop_A01G003858 and Pop_G11G077730, were confirmed to be localized in the nucleus.
Conclusions:
- The study provides insights into the evolutionary relationships of AP2/EREBP genes in poplar leaves.
- The identified genes, particularly those with decreasing expression, are potential targets for future research on leaf development regulation.
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