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Related Concept Videos

Inhibitors of Viral Protein Synthesis01:30

Inhibitors of Viral Protein Synthesis

73
Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...
73

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Related Experiment Video

Updated: May 5, 2026

Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus
11:28

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African Swine Fever Virus MGF 360-2L Disrupts Host Antiviral Immunity Based on Transcriptomic Analysis.

Taoqing Zhang1,2, Xiaodong Qin1,2, Sujie Dong1,2

  • 1State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.

Vaccines
|September 27, 2025
PubMed
Summary

African swine fever virus MGF 360-2L is a late gene crucial for regulating viral protein expression and host immune response. Its absence disrupts replication-essential genes and impacts Type I interferon signaling pathways.

Keywords:
African swine fever virusMGF 360-2LRNA-Seqhost antiviral immunityrecombinant virus functional analysis

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Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • African swine fever virus (ASFV) multi-gene family (MGF) 360 proteins are key to immune evasion, replication, and virulence.
  • The specific functions of many ASFV MGF proteins remain incompletely understood.

Purpose of the Study:

  • To investigate the functional role of the ASFV MGF 360-2L protein.
  • To elucidate the impact of MGF 360-2L on viral gene expression and host immune response.

Main Methods:

  • Transcriptional kinetics analysis to determine MGF 360-2L expression timing.
  • Transcriptomic profiling of ASFV-infected cells lacking MGF 360-2L (ΔMGF 360-2L).
  • Gene Ontology (GO) and KEGG pathway enrichment analyses of differentially expressed genes (DEGs).

Main Results:

  • MGF 360-2L exhibits late gene expression kinetics, similar to the p72 gene (B646L).
  • Absence of MGF 360-2L led to significant differential gene expression at 12 and 24 hours post-infection.
  • DEGs were primarily enriched in Type I interferon (IFN-I) signaling pathways.
  • MGF 360-2L is essential for the stable expression of replication-associated genes E199L and E301R.

Conclusions:

  • ASFV MGF 360-2L functions as a late-acting gene.
  • MGF 360-2L plays a critical role in the precise regulation of viral protein synthesis.
  • This protein modulates the host's immune response during ASFV infection.