Plasma-Engineered PDRN: Surface Charge Neutralization and Nanosizing Enhance Uptake and Regeneration Potential

Area of Science:

• Biomaterials Science
• Dermatology
• Cosmetic Science

Background:

• Polydeoxyribonucleotide (PDRN) possesses regenerative and anti-aging properties valuable in dermatology and cosmetics.
• High molecular weight and anionic charge limit topical PDRN skin penetration.
• Novel methods are needed to enhance PDRN's efficacy for topical applications.

Purpose of the Study:

• To investigate the potential of nitrogen-oxygen plasma treatment to improve PDRN's physicochemical properties.
• To evaluate the in vitro efficiency of plasma-treated PDRN for enhanced topical delivery.

Main Methods:

• Nitrogen-oxygen plasma treatment was applied to PDRN.
• Physicochemical properties (surface charge, hydrodynamic size) were characterized.
• In vitro cell migration assays were performed to assess efficacy.

Main Results:

• Plasma treatment attenuated PDRN's surface charge and decreased its hydrodynamic size.
• Improved cellular uptake of PDRN was observed post-treatment.
• Plasma-treated PDRN demonstrated markedly increased cell migration activity.

Conclusions:

• Plasma treatment effectively modifies PDRN, enhancing its suitability for cosmetic applications.
• This approach offers a potential strategy for adapting other high-molecular-weight bioactives for topical delivery.
• Plasma-treated PDRN represents a promising ingredient for advanced skincare formulations.