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Area of Science:

  • Glycobiology
  • Virology
  • Pharmacology

Background:

  • Core fucose is a critical glycan in Hepatitis B virus (HBV) infection.
  • Previous studies indicated lectins' potential in targeting HBV infection.

Purpose of the Study:

  • To investigate the inhibitory effect of Pholiota squarrosa lectin (PhoSL) on HBV infection in normal human hepatocytes.
  • To explore the mechanism and therapeutic potential of PhoSL against HBV.

Main Methods:

  • Utilized a normal human hepatocyte model for HBV infection.
  • Administered PhoSL during and after HBV infection to assess its impact.
  • Analyzed HBe antigen production, HBV cccDNA levels, and EGFR phosphorylation.

Main Results:

  • PhoSL inhibited HBe antigen production and HBV cccDNA in a concentration-dependent manner.
  • PhoSL suppressed HBe antigen production even when administered post-infection.
  • The mechanism involves binding to the HBV receptor and inhibiting EGFR phosphorylation, thus blocking viral entry.

Conclusions:

  • PhoSL demonstrates significant inhibitory effects against HBV infection in hepatocytes.
  • PhoSL shows potential as a clinically applicable carbohydrate therapy for HBV due to its stability and synthesizability.
  • Further research is needed to fully elucidate additional mechanisms of PhoSL's anti-HBV activity.