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Isolation and Characterization of Equine Lymph Node Endothelial Cells.

Tomas Lugo1, Stephanie Myers1, Thu Annelise Nguyen1

  • 1School of Veterinary Medicine, Texas Tech University, Amarillo, TX 79106, USA.

Veterinary Sciences
|September 27, 2025
PubMed
Summary

Researchers developed a new equine cell culture from horse lymph nodes. This novel in vitro model advances veterinary research for drug testing and studying disease mechanisms in horses.

Keywords:
endothelial cellsequinelymph nodesmultisystemic eosinophilic epitheliotropic diseaseprimary cells

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Area of Science:

  • Veterinary Medicine
  • Cell Biology
  • Genomics

Background:

  • In vitro models are crucial for biological research but equine models are scarce.
  • Existing models do not fully represent equine physiology.

Purpose of the Study:

  • To establish a novel primary cell culture from equine lymph nodes.
  • To characterize these cells for potential in vitro applications.
  • To address the lack of specialized equine models in research.

Main Methods:

  • Primary cell culture established from lymph node tissue of a horse with multisystemic eosinophilic epitheliotropic disease.
  • Growth curve analysis and optimization of culture conditions.
  • Immunofluorescence assay for biomarker detection (CD31, ZO-1, CD79, Beta-catenin, E-cadherin, LYE-1).
  • Whole-genome sequencing for species confirmation and variation analysis against EquCab3.0.

Main Results:

  • Successfully established primary equine cell cultures from lymph nodes.
  • Immunofluorescence confirmed endothelial origin of the cells.
  • Whole-genome sequencing verified equine origin and provided genomic variation data.
  • Optimal growth conditions for the primary cells were determined.

Conclusions:

  • This study reports the first establishment of primary equine lymph node cells.
  • These cells represent a valuable new in vitro model for equine research.
  • The model can be utilized for drug response, molecular pathway, and environmental effect testing in horses.