Related Experiment Video

Updated: May 14, 2026

Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis
07:34

Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis

Published on: April 16, 2019

Bacterial profiling and antibiotic resistance patterns in urinary tract infections: a microbiological analysis from

Zahid Ullah1, Junaid Asghar2, Nighat Aziz3

  • 1Department of Pathology, Gomal Medical College, Dera Ismail Khan, 29050, Pakistan. zahidwazir150@hotmail.com.

BMC Infectious Diseases
|September 27, 2025
PubMed
Abstract

Insights

Urinary tract infections (UTIs) are common, especially in D.I.Khan, Pakistan. This study found E. coli as the main pathogen, with high resistance to common antibiotics but susceptibility to imipenem, nitrofurantoin, and fosfomycin.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Public Health

Background:

  • Urinary tract infections (UTIs) are a global health issue, particularly prevalent in Pakistan.
  • The district of Dera Ismail Khan (D.I.Khan) faces significant challenges with UTIs.
  • Understanding the local bacterial epidemiology and antibiotic resistance patterns is crucial for effective management.

Purpose of the Study:

  • To determine the frequency of bacterial isolates causing UTIs in D.I.Khan.
  • To analyze the antibiotic susceptibility profiles of these bacterial isolates.
  • To inform local treatment guidelines and public health strategies.

Main Methods:

  • A prospective study conducted from January 2022 to November 2024 in D.I.Khan.
  • Urine culture and sensitivity testing performed on 610 individuals.
  • Aseptic collection and processing of all urine specimens.

Main Results:

  • 260 out of 610 (42.6%) individuals had positive bacterial urine cultures.
  • Escherichia coli (E. coli) was the most common pathogen (59.6%), prevalent in older adults (>60 years).
  • High resistance was observed against amoxicillin-clavulanic acid and ampicillin (100%), while imipenem, nitrofurantoin, and fosfomycin showed effectiveness.

Conclusions:

  • The study reveals the predominant bacterial pathogens and concerning antimicrobial resistance trends in UTIs within D.I.Khan.
  • Effective treatment options include imipenem, nitrofurantoin, and fosfomycin.
  • Regular surveillance and updated empirical treatment strategies are essential for combating UTIs.
Keywords:
Antibiotic susceptibilityAntimicrobial resistanceD.I.KhanSouth AsiaUrinary tract infections

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