Related Experiment Video
Updated: Jan 6, 2026

Aseptic Laboratory Techniques: Volume Transfers with Serological Pipettes and Micropipettors
Published on: May 31, 2012
Operating room nurses' experiences of maintaining sterile technique: a qualitative study.
Nurten Taşdemir1, Dilek Yildirim Tank2
1Nursing Department, Faculty of Health Sciences, Zonguldak Bülent Ecevit University, Zonguldak, Turkey. nurten.tasdemir@beun.edu.tr.
Operating room (OR) nurses face significant challenges in maintaining sterile technique due to systemic issues and interpersonal dynamics. Improving training, communication, and support systems is crucial for enhancing patient safety and OR practices.
Area of Science:
- Nursing
- Surgical Safety
- Healthcare Management
Background:
- Operating room (OR) nurses are vital for preventing surgical site infections (SSIs) through sterile technique.
- Persistent challenges in maintaining sterility arise from systemic factors and interpersonal dynamics.
- Understanding OR nurses' experiences is key to improving patient safety.
Purpose of the Study:
- To explore the experiences of OR nurses in maintaining sterile technique.
- To identify challenges, emotional burdens, and teamwork dynamics affecting sterility.
- To gather recommendations for enhancing sterile technique practices.
Main Methods:
- Qualitative descriptive study utilizing semi-structured interviews.
- 12 OR nurses from a Turkish university hospital participated.
- Content analysis was applied to transcribed interview data.
Main Results:
- Key themes included systemic challenges (e.g., rushed schedules, staff shortages), teamwork issues (e.g., non-compliance, communication breakdowns), and emotional burden (e.g., stress, anxiety).
- Nurses reported a strong sense of moral responsibility for patient safety.
- Recommendations focused on structured training, improved communication, and appropriate staff selection.
Conclusions:
- OR nurses encounter substantial obstacles in maintaining sterile technique.
- Organizational inefficiencies, interpersonal conflicts, and emotional stress significantly impact sterile practices.
- Enhancements in support systems, communication, and training are recommended to improve sterile technique and patient safety.
Related Concept Videos
Key Techniques in Microbiology
Tracheostomy Care I: Pre-procedural Steps
Required Equipment
The equipment necessary for tracheostomy care includes:
Tracheostomy Care II: Procedure
Step 1: Perform hand hygiene, and put on personal protective equipment: gown, gloves, mask...
Tracheostomy Suctioning II: Procedure
Sputum Studies II: Culture and Sensitivity
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...
Asepsis

