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Updated: Jan 16, 2026
Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
Published on: May 19, 2023
Protocol for stromal vascular fraction isolation from inguinal subcutaneous white adipose tissue and beige adipocyte
Qiqi Tang1, Juan Xu2, Xinyu Yang2
1Department of Pharmacology, State Key Laboratory of Experimental Hematology, Tianjin Key Laboratory of Inflammatory Biology, The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, NHC Key Laboratory of Hormones and Development, Chu Hsien-I Memorial Hospital and Tianjin Institute of Endocrinology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China; Department of Physiology and Pathophysiology, Tianjin Key Laboratory of Cell Homeostasis and Major Diseases, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China.
Abstract:
Research on inducing beige adipocytes to combat obesity and its comorbidities is growing, with stromal vascular fraction (SVF) from white adipose tissue showing potential to differentiate into both white and beige adipocytes. Here, we present a protocol for isolating the SVF from the iWAT (inguinal white adipose tissue) of mice and differentiating them into mature beige adipocytes. This protocol can be used to analyze the translational regulation and metabolic processes of beige adipocytes in vitro. For complete details on the use and execution of this protocol, please refer to Xu et al.1.
