Related Experiment Video
Updated: Jan 16, 2026

Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues
Published on: February 27, 2021
Protocol for immunofluorescence imaging of epitope-tagged protein in Arabidopsis shoot apical meristem
Hiroshi Takagi1, Naoyuki Uchida2, Takato Imaizumi3
1Bioscience and Biotechnology Center, Nagoya University, Nagoya 464-8601, Japan; Institute for Advanced Research (IAR), Nagoya University, Nagoya 464-8601, Japan.
Abstract:
Proteins expressed in or translocated to the shoot apical meristem (SAM) often play pivotal roles in regulating plant development. Here, we present a step-by-step protocol for immunofluorescence imaging to analyze epitope (FLAG)-tagged proteins in the Arabidopsis SAM. This technique may be suitable to visualize mobile proteins because small epitope tags are less disruptive than a relatively large fluorescent protein fusion. For complete details on the use and execution of this protocol, please refer to Takagi et al.1.
More Related Videos
08:21Detection of Protein Interactions in Plant using a Gateway Compatible Bimolecular Fluorescence Complementation BiFC System
Published on: September 16, 2011
12:01Long-term, High-resolution Confocal Time Lapse Imaging of Arabidopsis Cotyledon Epidermis during Germination
Published on: December 31, 2012