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A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
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Enhancing Chitosanase Activity via Directed Evolution and Its Molecular Mechanisms.

Yao Chen1, Xiangyu Zi1,2, Min Chen1

  • 1Taizhou Key Laboratory of Biomass Functional Materials Development and Application, School of Life Science, Taizhou University, Taizhou 318000, China.

Journal of Agricultural and Food Chemistry
|September 27, 2025
PubMed
Summary

Directed evolution significantly enhanced chitosanase activity, yielding a variant M1 with 812-fold higher performance. This engineered enzyme efficiently produces chitooligosaccharides (COS) from fungal chitosan, offering a valuable industrial tool.

Keywords:
Streptomyces sp. N174chitooligosaccharideschitosanasedirected evolution

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Area of Science:

  • Enzymology and protein engineering
  • Biotechnology and industrial microbiology

Background:

  • Chitosanases are crucial enzymes for degrading chitosan into valuable chitooligosaccharides (COS).
  • Optimizing chitosanase performance is essential for efficient and scalable COS production.

Purpose of the Study:

  • To enhance the performance of a rationally designed chitosanase (mSsCsn46) through directed evolution.
  • To develop a highly efficient enzymatic tool for industrial chitooligosaccharide production.

Main Methods:

  • Directed evolution of chitosanase SsCsn46 fused with an OmpA signal peptide.
  • Enzyme activity assays and characterization of the evolved variant (M1).
  • Molecular dynamics simulations to elucidate structural changes and substrate-binding interactions.

Main Results:

  • The evolved variant M1 exhibited an 812-fold increase in enzyme activity (1680 U/mg) compared to the parent enzyme.
  • M1 produced 142 g/L of COS with 89.9% conversion within 3 hours via continuous substrate feeding.
  • M1 rapidly converted fungal chitosan to COS with 95.1% conversion in just 30 minutes.

Conclusions:

  • Directed evolution effectively remodeled chitosanase for significantly improved COS production efficiency.
  • The engineered chitosanase M1 presents a potent enzymatic solution for industrial-scale COS manufacturing.
  • Structural insights from molecular dynamics simulations explain the enhanced enzyme performance.