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CRISPR/Cas as a tool to overcome drug resistance in cancer: From challenge to opportunity

Bashdar Mahmud Hussen1, Snur Rasool Abdullah2, Hazha Jamal Hidayat3

  • 1Department of Clinical Analysis, College of Pharmacy, Hawler Medical University, Kurdistan Region, Erbil, Iraq; Department of Medical Laboratory Science, College of Health Sciences, Lebanese French University, Kurdistan Region, Erbil, Iraq.

Molecular and Cellular Probes
|September 28, 2025
PubMed

Insights

CRISPR-Cas gene editing offers a novel approach to understanding and overcoming cancer drug resistance by precisely targeting resistance genes. Further advancements in delivery and specificity are crucial for clinical application.

Area of Science:

  • Oncology
  • Genetics
  • Biotechnology

Background:

  • Drug resistance is a major obstacle in cancer therapy, leading to treatment failure and disease progression.
  • Mechanisms of drug resistance, including genetic and epigenetic factors, are complex and require further elucidation.
  • CRISPR-Cas gene editing technology presents a powerful tool for investigating these mechanisms.

Purpose of the Study:

  • To review the application of CRISPR-Cas gene editing in understanding cancer drug resistance.
  • To explore how CRISPR-Cas can identify and target genes associated with drug resistance.
  • To discuss the potential of CRISPR-Cas in restoring drug sensitivity and developing new therapeutic strategies.

Main Methods:

  • Review of current literature on CRISPR-Cas applications in cancer drug resistance research.
  • Analysis of CRISPR-Cas based approaches for dissecting molecular mechanisms of resistance.
  • Evaluation of strategies for enhancing CRISPR-Cas efficacy and safety.

Main Results:

  • CRISPR-Cas enables precise genetic modifications to gain insights into drug resistance.
  • The technology shows promise in identifying resistance-associated genes and developing targeted therapies.
  • Limitations include delivery challenges, off-target effects, and specificity issues.

Conclusions:

  • CRISPR-Cas technology holds significant potential for addressing cancer drug resistance through targeted genetic interventions.
  • Ongoing research focuses on improving delivery systems, guide RNA design, and specificity.
  • Further advancements are necessary for successful clinical translation and integration into cancer treatment regimens.
Keywords:
CRISPR limitationsCRISPR/CasCancerCancer therapyDrug resistance

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