Risk Evaluation of Verticillium Wilt on Cabbage Grown in Soil Reused from Sediment Basins

There is a growing demand for the reuse of sediment basin soil in cabbage fields; however, reusing soil poses a potential challenge of spreading Verticillium wilt to the fields via pathogen-infested sediments. We evaluated the density of the Verticillium wilt pathogen in sediment basin soil using a quantitative nested real-time polymerase chain reaction assay and its incidence using pot cultivation tests. We detected low pathogenic DNA levels in the sediment, coupled with a low Verticillium wilt incidence and severity in the pot experiment, indicating a low risk of spreading Verticillium wilt with the reuse of sediment basin soil.