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High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

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Published on: August 3, 2011

Integrated Microfluidic System for Rapid 89-Plex Y-SNP Profiling: Development and Forensic Validation.

Yongjie Li^1,2, Li Jiang², Qingyang Liu^1,2

¹School of Criminal Investigation, People's Public Security University of China, Beijing, China.

Electrophoresis

|September 29, 2025

Summary

This summary is machine-generated.

A new microfluidic system offers rapid, on-site Y-SNP genotyping for forensic genetics. This portable device enables automated analysis, improving efficiency for tasks like ancestry inference and mixture interpretation.

Keywords:

forensic genetics | haplogroup analysis | microfluidic chip | rapid on‐site detection | Y‐chromosomal single‐nucleotide polymorphism (Y‐SNP) genotyping

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Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

Area of Science:

Forensic Genetics
Microfluidics
Molecular Biology

Background:

Current Y-chromosomal single-nucleotide polymorphism (Y-SNP) detection methods are often cumbersome and not field-adaptable.
There is a need for portable, automated solutions for Y-SNP analysis in forensic applications.

Purpose of the Study:

To develop and validate an 89-plex microfluidic Y-SNP system for rapid, on-site genotyping.
To create a fully automated workflow from sample to result, enhancing forensic analysis capabilities.

Main Methods:

Development of a microfluidic system with disposable DNA extraction/amplification and capillary electrophoresis chips.
Integration of in situ lyophilized reagents for long-term storage and a portable device for automation.
Design of two panels (AIYSNP42 and AIYSNP47) based on the ISOGG phylogenetic tree for multi-level haplogroup resolution.

Main Results:

Achieved a fully automated "sample-in-answer-out" workflow, completing 89-locus Y-SNP genotyping in 139 minutes.
Demonstrated high sensitivity (2.5 ng DNA), success rate (93.6% across 94 samples), and robustness against inhibitors and DNA degradation.
Showcased excellent reproducibility (CV < 0.5%) and reliable detection of male DNA in mixtures (≥2% in male-female, ≥33% in male-male).

Conclusions:

The developed microfluidic system significantly reduces reliance on traditional laboratory workflows.
Enables rapid, on-site Y-SNP analysis for diverse forensic applications including pedigree tracing, ancestry inference, and mixture interpretation.