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Updated: May 1, 2026
Identification of Circular RNAs using RNA Sequencing
Published on: November 14, 2019
Visual enumeration of circRNA in serum using a biserrate DNA-modified AuNP SPR probe
Yuanzhao Shen1, Ran Wang1, Xin Zhou2,3,4
1Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
Abstract:
This study developed a 60 nm biserrate DNA-modified Au nanoparticle (AuNP) platform for rapid circRNA detection using dark-field microscopy (DFM). Compared with other Au nanostructures, spherical AuNPs exhibited superior stability and sensitivity, achieving a detection limit of ∼0.02 fM for circHECTD1 within 40 min, representing about a 100-fold improvement in sensitivity over the qPCR method for this target. This approach enables fast, cost-effective, and highly sensitive circRNA analysis without PCR amplification.
