Testing for heavy metals in drinking water collected from Dog Aging Project participants
Courtney L Sexton1, Janice O'Brien1, Justin Lytle2
1Department of Population Health Sciences, Virginia-Maryland College of Veterinary Medicine, Virginia Tech, Blacksburg, Virginia, United States of America.
PLOS Water
|September 29, 2025
Summary
This pilot study found detectable levels of heavy metals in dogs' drinking water, with some exceeding Environmental Protection Agency (EPA) safety limits. These findings suggest dogs may serve as sentinels for potential human health risks from contaminated water.
Area of Science:
- Environmental Science
- Veterinary Medicine
- Toxicology
Background:
- Groundwater contamination by heavy metals poses risks to drinking water quality.
- The Dog Aging Project (DAP) provided a unique cohort of dogs not connected to municipal water supplies.
- Understanding metal exposure in canine drinking water is crucial for animal and potentially human health.
Purpose of the Study:
- To assess the presence and levels of 28 metals, including EPA-regulated heavy metals, in the drinking water of DAP dogs.
- To investigate associations between water source characteristics, metal concentrations, and canine disease development.
- To evaluate the feasibility of at-home water sampling for detecting metal contaminants.
Main Methods:
- Pilot study involving 200 DAP dog owners across 10 selected states.
- Analysis of 28 metals in home-collected drinking water samples.
- Regression modeling to explore relationships between water quality, metal levels, and health outcomes.
Main Results:
- All tested metals were detected in the drinking water samples.
- 126 instances exceeded EPA Maximum Contaminant Levels (MCLs) or health guidance levels for specific metals (e.g., arsenic, lead, copper).
- Potential associations were found between titanium/chromium and specific canine health conditions.
Conclusions:
- At-home sampling is effective for detecting metal contaminants in canine drinking water.
- Elevated metal levels in drinking water are prevalent and may be linked to canine health issues.
- Dogs can serve as valuable sentinels for assessing environmental drinking water quality and associated health risks for humans.


