Fluorescent Probes for Endogenous Hydrogen Sulfide: Advances and Challenges

Area of Science:

• Biochemistry
• Chemical Biology
• Molecular Imaging

Background:

• Hydrogen sulfide (H₂S) is a crucial endogenous signaling molecule involved in various physiological and pathological processes.
• Dysregulation of H₂S is linked to diseases, including cancer, making its detection vital.
• Fluorescent probes are powerful tools for monitoring H₂S in biological systems.

Purpose of the Study:

• To review fluorescent probes for detecting endogenous H₂S without external stimulators.
• To focus on probes suitable for in vitro and in vivo applications from 2014-2024.
• To provide background for developing novel H₂S-sensing probes.

Main Methods:

• Literature review of scientific publications from 2014 to 2024.
• Focus on fluorescent probes designed for endogenous H₂S detection.
• Analysis of probes used for both in vitro and in vivo studies.

Main Results:

• Identified and summarized key fluorescent probes for endogenous H₂S detection.
• Highlighted probes that do not require external stimuli for activation.
• Covered applications in various biological contexts, including disease models.

Conclusions:

• There is a growing need for fluorescent probes that can detect endogenous H₂S without external stimulation.
• Such probes are essential for accurate in vivo tracking of H₂S in pathological conditions.
• This review provides a foundation for future development of advanced H₂S-monitoring tools.