Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

9.8K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
9.8K
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

8.6K
The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
8.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

JIN-A02, a Mutant-Selective Fourth-Generation EGFR Inhibitor, Overcomes C797S-Mediated Resistance and Demonstrates Intracranial Activity in NSCLC.

Clinical cancer research : an official journal of the American Association for Cancer Research·2026
Same author

Detection and Genetic Characterization of <i>Enterocytozoon hepatopenaei</i> in Giant Freshwater Prawn (<i>Macrobrachium rosenbergii</i>) Imported into South Korea.

Animals : an open access journal from MDPI·2025
Same author

Protein Microplastic Coronation Complexes Trigger Proteome Changes in Brain-Derived Neuronal and Glial Cells.

Environmental science & technology·2025
Same author

First report of iron-overload myopathy due to secondary hemochromatosis in a dog.

Journal of veterinary science·2025
Same author

RETRACTED: Jeong et al. A Phase III Head-to-Head Study to Compare the Efficacy and Safety of Fexuprazan and Esomeprazole in Treating Patients with Erosive Esophagitis. <i>J. Clin. Med.</i> 2024, <i>13</i>, 3262.

Journal of clinical medicine·2024
Same author

Genomic and Pathological Characterization of Acute Hepatopancreatic Necrosis Disease (AHPND)-Associated Natural Mutant <i>Vibrio parahaemolyticus</i> Isolated from <i>Penaeus vannamei</i> Cultured in Korea.

Animals : an open access journal from MDPI·2024

Related Experiment Video

Updated: Jan 16, 2026

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

8.0K

Veratramine Inhibits Human Non-Small Cell Lung Cancer Cell Growth by Inhibiting the Hedgehog Signaling Pathway.

Seoung-Woo Lee1,2, Hee-Yeon Kim1,3, Wansoo Kim4

  • 1Core Protein Resources Center, Daegu-Gyeongbuk Institute of Science and Technology (DGIST), Daegu, Republic of Korea.

Journal of Pharmacopuncture
|September 29, 2025
PubMed
Summary

Veratramine, a natural compound, was found to inhibit non-small cell lung cancer (NSCLC) cell growth and migration. This natural steroidal alkaloid also promotes apoptosis and delays the cell cycle by downregulating the Hedgehog signaling pathway.

Keywords:
Hedgehog signaling pathwaylung cancernon-small cell lung carcinomaphytomedicineveratramine

More Related Videos

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor
07:16

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor

Published on: September 13, 2018

20.6K
Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment
11:13

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Published on: June 9, 2023

2.3K

Related Experiment Videos

Last Updated: Jan 16, 2026

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

8.0K
Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor
07:16

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor

Published on: September 13, 2018

20.6K
Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment
11:13

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Published on: June 9, 2023

2.3K

Area of Science:

  • Pharmacology
  • Oncology
  • Molecular Biology

Background:

  • Non-small cell lung cancer (NSCLC) remains a leading cause of cancer mortality globally.
  • There is a critical need for novel therapeutic strategies to combat NSCLC.
  • Veratramine, a steroidal alkaloid from lily plants, shows potential anti-cancer properties, but its role in NSCLC is not well-defined.

Purpose of the Study:

  • To investigate the effects of veratramine on NSCLC cell lines.
  • To elucidate the underlying molecular mechanisms of veratramine's action in NSCLC.

Main Methods:

  • NSCLC cell lines (A549, NCI-H358, NCI-H1299) were treated with varying concentrations of veratramine.
  • Cell viability, apoptosis, and cell cycle progression were assessed using CCK-8 and flow cytometry.
  • Protein expression, including components of the Hedgehog signaling pathway, was analyzed via immunoblotting.

Main Results:

  • Veratramine significantly reduced NSCLC cell viability and migration.
  • Treatment with veratramine induced apoptosis and caused cell cycle arrest.
  • The Hedgehog (Hh) signaling pathway was downregulated, evidenced by decreased gli1 expression and reduced cell cycle-related proteins.

Conclusions:

  • Veratramine exhibits anti-cancer effects against NSCLC by suppressing cell growth and migration.
  • Inhibition of the Hedgehog signaling pathway is a key mechanism by which veratramine acts.
  • Veratramine demonstrates potential as a therapeutic agent for NSCLC treatment.