Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Polymer Molecular Weight Influences Cancer Cell Surface Retention and Cytokine Presentation by Layer-by-Layer Nanoparticles.

ACS nano·2026
Same author

Engineering nanoparticle surface chemistry for antigen-presenting cell targeting improves specificity and safety of TLR3 agonist cancer immunotherapy.

bioRxiv : the preprint server for biology·2026
Same author

Vaccination elicits HIV broadly neutralizing antibodies in primates.

Nature·2026
Same author

Enhanced B cell priming induces broadly neutralizing HIV-1 apex antibodies.

Nature·2026
Same author

Engineering drug-responsive replication machinery for precise control of self-amplifying RNA.

Nature biomedical engineering·2026
Same author

Rapid boosting increases germinal center responses to sequential vaccines.

Nature immunology·2026

Related Experiment Video

Updated: Jan 16, 2026

Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly
10:17

Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly

Published on: November 4, 2021

3.6K

High-Throughput Microfluidic-Mediated Assembly of Layer-by-Layer Nanoparticles.

Ivan S Pires1,2, Ezra Gordon3, Heikyung Suh3

  • 1Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, 500 Main Street, Cambridge, Massachusetts 02139, United States.

Advanced Functional Materials
|September 29, 2025
PubMed
Summary

Microfluidic mixing offers a scalable method for creating layer-by-layer nanoparticles (LbL-NPs) for drug delivery. This technique eliminates purification steps, increasing efficiency and throughput for clinical translation.

Keywords:
drug deliverylayer-by-layermicrofluidicsnanoparticlesscale-uptargeted

More Related Videos

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles
11:13

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles

Published on: March 13, 2016

11.2K
A Technique to Functionalize and Self-assemble Macroscopic Nanoparticle-ligand Monolayer Films onto Template-free Substrates
08:09

A Technique to Functionalize and Self-assemble Macroscopic Nanoparticle-ligand Monolayer Films onto Template-free Substrates

Published on: May 9, 2014

11.4K

Related Experiment Videos

Last Updated: Jan 16, 2026

Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly
10:17

Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly

Published on: November 4, 2021

3.6K
Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles
11:13

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles

Published on: March 13, 2016

11.2K
A Technique to Functionalize and Self-assemble Macroscopic Nanoparticle-ligand Monolayer Films onto Template-free Substrates
08:09

A Technique to Functionalize and Self-assemble Macroscopic Nanoparticle-ligand Monolayer Films onto Template-free Substrates

Published on: May 9, 2014

11.4K

Area of Science:

  • Nanotechnology
  • Materials Science
  • Biomedical Engineering

Background:

  • Surface modification of nanoparticles (NPs) using layer-by-layer (LbL) assembly is crucial for developing targeted drug delivery systems.
  • Existing LbL methods often require time-consuming purification, limiting scalability and clinical translation.
  • A need exists for a simplified, robust, and scalable synthesis of LbL-NPs.

Purpose of the Study:

  • To develop a novel, scalable microfluidic (MCF) mixing method for polymer deposition onto NPs for LbL assembly.
  • To demonstrate that MCF-based LbL-NP synthesis can eliminate the need for post-assembly purification.
  • To validate the efficacy of MCF-assembled LbL-NPs compared to traditional methods.

Main Methods:

  • Utilized commercially available bifurcating mixer microfluidic cartridges for mixing NPs with polyelectrolytes.
  • Employed titrated polymer-to-NP weight equivalent ratios for electrostatic assembly, avoiding excess polymer.
  • Demonstrated LbL film assembly on various NP core substrates and with different polymer chemistries.

Main Results:

  • MCF mixing enabled robust LbL electrostatic assembly without requiring excess polymer or purification steps.
  • This MCF approach significantly increased LbL-NP throughput and reduced NP loss.
  • IL-12-loaded liposomal NPs synthesized via MCF showed equivalent in vitro and in vivo efficacy to traditionally produced LbL-NPs.

Conclusions:

  • Microfluidic mixing presents a scalable, efficient, and robust platform for synthesizing LbL-NPs for drug delivery.
  • The elimination of purification steps simplifies the process and enhances its suitability for clinical translation.
  • MCF technology is versatile, applicable to diverse NP cores and polymer combinations for advanced nanomedicine applications.