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Updated: Jan 16, 2026

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
Published on: June 27, 2014
Time-Resolved Study of Light-Induced Ground-State Proton Transfer from an Acidic Medium to 4‑Nitrophenolate
Leandro Scorsin1, René A Nome2, Ricardo F Affeldt3
1Instituto de Química, Universidade Federal do Rio Grande do Sul-UFRGS. 91501-970 Porto Alegre, Rio Grande do Sul, Brasil.
None:
This work investigates the transient laser-induced formation of 4-nitrophenolate in the ground electronic state and its subsequent proton transfer with acetic acid and water. Laser flash photolysis in the UV-vis region revealed the presence of a deprotonated transient species even at weakly acidic pH. We measured the photoinitiated ground state protonation and deprotonation rate constants of 4-NPO-/4-NPOH as a function of acetic acid, pH, and temperature. This study demonstrates a simple approach to analyzing fast competing bimolecular proton transfer reactions under nonequilibrium conditions in the ground state.
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