Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Separation of Sister Chromatids02:17

Separation of Sister Chromatids

4.4K
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
4.4K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

6.3K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
6.3K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.7K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.7K
Replication in Prokaryotes01:32

Replication in Prokaryotes

27.6K
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
27.6K
Replication in Prokaryotes02:35

Replication in Prokaryotes

96.9K
Overview
96.9K
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

10.0K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
10.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Membrane-associated effluxosomes coordinate multi-metal resistance in Mycobacterium tuberculosis.

The EMBO journal·2026
Same author

Two OB-fold proteins from a Gram-positive conjugative element engage in relaxosome assembly and DNA processing.

Nucleic acids research·2025
Same author

Anyon Braiding on the Single Edge of a Fractional Quantum Hall State.

Physical review letters·2025
Same author

PacL-organized membrane-associated effluxosomes coordinate multi-metal resistance in <i>Mycobacterium tuberculosis</i>.

bioRxiv : the preprint server for biology·2025
Same author

Future Directions of the Prokaryotic Chromosome Field.

Molecular microbiology·2025
Same author

Thermoelectric properties of a quantum dot attached to normal metal and topological superconductor.

Scientific reports·2025

Related Experiment Video

Updated: Jan 16, 2026

Synchronization of Caulobacter Crescentus for Investigation of the Bacterial Cell Cycle
08:02

Synchronization of Caulobacter Crescentus for Investigation of the Bacterial Cell Cycle

Published on: April 8, 2015

12.4K

The ParB-CTP cycle activates phase separation in bacterial DNA segregation.

Linda Delimi1, Perrine Revoil2, Hicham Sekkouri Alaoui2

  • 1Laboratoire Charles Coulomb (L2C), Univ. Montpellier, CNRS, Montpellier, 34090, France.

Nucleic Acids Research
|September 29, 2025
PubMed
Summary

The ParB-CTP cycle acts as a molecular switch, enhancing protein interactions to trigger phase transitions for bacterial chromosome segregation. This mechanism explains the formation of essential cellular condensates.

More Related Videos

BEST: Barcode Enabled Sequencing of Tetrads
12:59

BEST: Barcode Enabled Sequencing of Tetrads

Published on: May 1, 2014

10.6K
Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
08:13

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

477

Related Experiment Videos

Last Updated: Jan 16, 2026

Synchronization of Caulobacter Crescentus for Investigation of the Bacterial Cell Cycle
08:02

Synchronization of Caulobacter Crescentus for Investigation of the Bacterial Cell Cycle

Published on: April 8, 2015

12.4K
BEST: Barcode Enabled Sequencing of Tetrads
12:59

BEST: Barcode Enabled Sequencing of Tetrads

Published on: May 1, 2014

10.6K
Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
08:13

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

477

Area of Science:

  • Cell biology
  • Biophysics
  • Molecular mechanisms of intracellular organization

Background:

  • Membraneless organelles form via phase transitions, crucial for cell function but poorly understood.
  • The bacterial ParABS system uses ParB proteins for chromosome segregation, with ParB binding CTP.
  • Existing models fail to explain ParB recruitment and phase separation dynamics.

Purpose of the Study:

  • To reconcile the ParB-CTP cycle with phase separation models.
  • To elucidate the mechanism of ParB-mediated condensate formation.
  • To understand the role of the ParB-CTP cycle in bacterial chromosome segregation.

Main Methods:

  • Numerical simulations of droplet formation.
  • Experimental analysis of ParB variants with disrupted CTP cycles.
  • Investigating ParB-CTP interactions and phase transitions.

Main Results:

  • The ParB-CTP cycle enhances ParB-ParB interactions, triggering phase transitions.
  • ParB variants unable to complete the CTP cycle fail to undergo phase separation.
  • A mechanistic framework for ParB-CTP-mediated phase transitions is established.

Conclusions:

  • The ParB-CTP cycle is a molecular switch controlling phase separation.
  • This mechanism explains the specific recruitment and assembly of ParB condensates.
  • Findings offer insights into spatial control of intracellular condensate formation.