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  11. The Complete Chloroplast Genomes Of Three Manglietia Species And Phylogenetic Insight Into The Genus Manglietia Blume

The Complete Chloroplast Genomes of Three Manglietia Species and Phylogenetic Insight into the Genus Manglietia Blume

Yuan Luo1, Wei Luo1, Tongxing Zhao1

  • 1School of Ecology and Environmental Science, Yunnan University, Kunming 650504, China.

Current Issues in Molecular Biology
|September 29, 2025

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View abstract on PubMed

Summary
This summary is machine-generated.

This study sequenced chloroplast genomes of three Manglietia species, revealing new phylogenetic insights and identifying key molecular markers for species identification. The findings clarify the infrageneric classification of Manglietia, a genus with significant economic and ornamental value.

Area of Science:

  • Botany
  • Genomics
  • Evolutionary Biology

Background:

  • The infrageneric classification of the Magnoliaceae family genus *Manglietia* is unclear due to subtle morphological differences and limited molecular data.
  • Accurate phylogenetic relationships are essential for species classification, genetic diversity assessment, and evolutionary studies within *Manglietia*.

Purpose of the Study:

  • To sequence, assemble, and annotate the chloroplast (cp) genomes of *Manglietia guangnanica*, *Manglietia hookeri*, and *Manglietia longirostrata*.
  • To clarify the phylogenetic relationships and infrageneric classification within the genus *Manglietia*.
  • To identify potential molecular markers for *Manglietia* species identification.

Main Methods:

  • Whole chloroplast genome sequencing, assembly, and annotation for three *Manglietia* species.
Keywords:
Manglietiachloroplast genomesinterspecific relationshipphylogeny

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  • Analysis of genome structure, repeat content, and codon usage bias.
  • Phylogenetic analysis using cp genome data.
  • Identification of highly variable regions for molecular marker development.

    • Main Results:

    • The cp genomes of the studied species exhibit canonical quadripartite structures and contain 133 genes.
    • Codon usage bias is primarily influenced by natural selection.
    • Phylogenetic analysis resolved four clades, including a newly discovered monotypic clade (Clade IV).
    • *petA*-*psbJ*, *rpl32*-*trnL*, and *ccsA*-*ndhD* regions are identified as promising molecular markers.
    • Morphological characteristics like hair presence on twigs, stipules, and leaf surfaces are important for distinguishing species within Clades I and III.
    • *M. guangnanica* and *M. calcarea* are confirmed as distinct species, and *M. longirostrata* is not a variety of *M. hookeri*.

    Conclusions:

    • This study significantly enriches the cp genome data for the genus *Manglietia*.
    • The findings provide novel insights into the infrageneric classification and evolutionary history of *Manglietia*.
    • The identified molecular markers will facilitate future taxonomic and phylogenetic research on *Manglietia* species.