Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Splicing01:32

RNA Splicing

60.4K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.4K
Alternative RNA Splicing02:18

Alternative RNA Splicing

24.7K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
24.7K
Alternative RNA Splicing02:18

Alternative RNA Splicing

4.8K
4.8K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

8.1K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
8.1K
Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

6.6K
6.6K
Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

3.3K
3.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A non-coding SNP in ELF3 alters ELF3β expression and confers adaptation of Arabidopsis to a continental climate.

Plant physiology·2026
Same author

A functional placenta-on-chip model for maternal-fetal transport.

Biofabrication·2026
Same author

Comparison of on-site versus NOAA's extreme precipitation intensity-duration-frequency estimates for six forest headwater catchments across the continental United States.

Stochastic environmental research and risk assessment : research journal·2026
Same author

DUSP11 is an RNA triphosphatase that limits PspCas13b activity by destabilizing gRNA abundance in mammalian cells.

Nucleic acids research·2026
Same author

Calling for Diversity: Improving Transfusion Safety Through High-Throughput Blood Group Microarray Genotyping.

Genomics, proteomics & bioinformatics·2026
Same author

Synthetic melanin as a bioinspired antioxidant: Modulating oxidative stress from 2D skin models to human skin validation.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same journal

Interplay between oxygen redox and interfacial stability of Li-rich positive electrodes in sulfide-based all-solid-state batteries.

Nature communications·2026
Same journal

Breaking dependence on melanisation imparts diversity to a dogmatic invasion strategy of phytopathogenic fungi.

Nature communications·2026
Same journal

Hydroxyl-rich nanocavities on perovskite enable nearly barrierless intramolecular hydrogen transfer for nitrate electroreduction to ammonia.

Nature communications·2026
Same journal

Household mobility responses to weather extremes in Kyrgyzstan.

Nature communications·2026
Same journal

Autonomous Motion Vision with Tri-bulk-heterojunctioned Organic Adaptation Transistor.

Nature communications·2026
Same journal

Tissue-adhesive hydrogel optical fiber for peripheral optogenetic neuromodulation.

Nature communications·2026
See all related articles

Related Experiment Video

Updated: Jan 16, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

5.4K

A basic framework to explain splice-site choice in eukaryotes.

Craig I Dent1,2, Stefan Prodic1,3, Aiswarya Balakrishnan1,4

  • 1School of Biological Sciences, Monash University, Clayton Campus, Melbourne, VIC, Australia.

Nature Communications
|September 29, 2025
PubMed
Summary
This summary is machine-generated.

Genetic variation significantly impacts gene splicing by altering splice-site strength. This study quantifies splice-site usage across species, revealing cis-acting variation as the primary driver and identifying hexamer rankings as a universal splicing rule.

More Related Videos

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

3.2K
ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

2.9K

Related Experiment Videos

Last Updated: Jan 16, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

5.4K
A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

3.2K
ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

2.9K

Area of Science:

  • Genetics
  • Molecular Biology
  • Bioinformatics

Background:

  • Splicing variations influence phenotypic traits and are linked to diseases.
  • Genetic and environmental factors affect splice-site strength, but the mechanisms are unclear.
  • Empirical quantification of splice-site usage across transcriptomes is lacking.

Purpose of the Study:

  • To quantify individual splice-site usage in Arabidopsis, Drosophila, and humans.
  • To map genetic variation influencing splice-site usage using genome-wide association studies (GWAS).
  • To identify conserved rules governing splice-site selection across eukaryotes.

Main Methods:

  • Quantification of splice-site usage as molecular phenotypes.
  • Performance of over 130,000 GWAS for splice-site usage variation.
  • Analysis of splice-site sequences (GT[N]4 or [N]4AG) and their usage patterns.

Main Results:

  • Cataloged genetic variation associated with changes in splice-site usage across transcriptomes.
  • Identified that most common, genetically controlled splicing variation is cis-acting, with no major trans hotspots.
  • Developed hexamer rankings based on sequence (GT[N]4 or [N]4AG) that effectively explain splice-site choice across species.

Conclusions:

  • Hexamer rankings provide a conserved, simple rule for splice-site selection in eukaryotes.
  • This study establishes a basis for understanding shared eukaryotic splicing logic.
  • Findings advance the understanding of how genetic variation impacts gene expression through splicing.