Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Studies III: Gastrointestinal Motility Studies and Virtual Colonoscopy01:26

Imaging Studies III: Gastrointestinal Motility Studies and Virtual Colonoscopy

This lesson explores three gastrointestinal imaging techniques: radionuclide testing, colonic transit studies, and virtual colonoscopy.
Radionuclide Testing
Radionuclide testing is a sophisticated medical technique for assessing gastrointestinal motility. It focuses on gastric emptying and colonic transit time. Radioactive markers track the movement of food through the digestive system, providing insights into gastrointestinal disorders.
In gastric emptying studies, a meal's liquid and solid...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multi-scale computational models of intervillous blood flow in fetal growth restriction: implications of anatomical changes on syncytiotrophoblast shear stress.

Placenta·2026
Same author

Anatomy of the Soft Palate and Its Role in Upper Airway Function: A Narrative Review.

Clinical anatomy (New York, N.Y.)·2026
Same author

Reply to Sanders et al.

American journal of physiology. Gastrointestinal and liver physiology·2026
Same author

Optical Mapping of Pacing-Elicited Slow Waves in the Swine Stomach: Role of Virtual Electrodes.

Neurogastroenterology and motility·2026
Same author

Computational modelling of the fetal circulation: what could cardiovascular system models teach us about the heart-placenta axis?

Computers in biology and medicine·2026
Same author

Ex vivo human placental imaging: Navigating modalities, scales, and analysis approaches to obtain fit-for-purpose data.

Placenta·2026

Related Experiment Video

Updated: Jul 9, 2026

Rat Mesentery Angiogenesis Assay
18:30

Rat Mesentery Angiogenesis Assay

Published on: June 18, 2011

14.2K

Detailed microCT imaging protocol for ex vivo rat stomachs with comparative analysis.

Savindi Wijenayaka1, Alys R Clark1, Dane Gerneke1

  • 1Auckland Bioengineering Institute, University of Auckland, Auckland, 1010, New Zealand.

Scientific Reports
|September 29, 2025
PubMed
Summary

This study optimized micro-computed tomography (μCT) for 3D rat stomach imaging. Phosphotungstic acid (PTA) staining for 7-9 weeks after 3 weeks of formalin fixation yielded the best results for anatomical analysis.

More Related Videos

Microperfusion Technique to Investigate Regulation of Microvessel Permeability in Rat Mesentery
12:48

Microperfusion Technique to Investigate Regulation of Microvessel Permeability in Rat Mesentery

Published on: September 12, 2015

10.1K
An Ex Vivo Method for Time-Lapse Imaging of Cultured Rat Mesenteric Microvascular Networks
06:54

An Ex Vivo Method for Time-Lapse Imaging of Cultured Rat Mesenteric Microvascular Networks

Published on: February 9, 2017

7.4K

Related Experiment Videos

Last Updated: Jul 9, 2026

Rat Mesentery Angiogenesis Assay
18:30

Rat Mesentery Angiogenesis Assay

Published on: June 18, 2011

14.2K
Microperfusion Technique to Investigate Regulation of Microvessel Permeability in Rat Mesentery
12:48

Microperfusion Technique to Investigate Regulation of Microvessel Permeability in Rat Mesentery

Published on: September 12, 2015

10.1K
An Ex Vivo Method for Time-Lapse Imaging of Cultured Rat Mesenteric Microvascular Networks
06:54

An Ex Vivo Method for Time-Lapse Imaging of Cultured Rat Mesenteric Microvascular Networks

Published on: February 9, 2017

7.4K

Area of Science:

  • Anatomy
  • Medical Imaging
  • Histology

Background:

  • Traditional 2D histology limits understanding of complex gastric structures.
  • Micro-computed tomography (μCT) offers high-resolution 3D imaging but requires effective soft tissue staining.

Purpose of the Study:

  • To establish optimal preparation and staining protocols for high-quality 3D anatomical reconstruction of the rat stomach using μCT.
  • To investigate the impact of fixation and staining durations on image quality and anatomical feature visualization.

Main Methods:

  • Rat stomachs underwent a five-stage preparation: fixation (2% neutral buffered formalin), dehydration (ethanol), staining (Lugol's solution or phosphotungstic acid-PTA), and μCT imaging.
  • Image quality was quantitatively assessed using signal-to-noise (SNR) and contrast-to-noise (CNR) ratios.

Main Results:

  • Phosphotungstic acid (PTA) staining significantly improved contrast-to-noise (CNR) and signal-to-noise (SNR) ratios compared to Lugol's solution.
  • Optimal PTA staining duration was 7-9 weeks, balancing contrast enhancement with minimized tissue damage.
  • Fixation duration had minimal impact, while prolonged staining led to tissue tearing and significant shrinkage (33.1% volume reduction).

Conclusions:

  • A protocol involving 3 weeks of 2% neutral buffered formalin fixation followed by 7-9 weeks of 2% PTA staining is recommended for high-quality μCT imaging of rat gastric tissue.
  • This optimized protocol facilitates detailed 3D anatomical analysis, aiding in the study of gastrointestinal disorders.