Related Experiment Video

Updated: Jan 16, 2026

Author Spotlight: Combining Proximity Ligand Assay with Gamma-H2AX Staining to Characterize Protein Interactions in DNA Damage Response
09:39

Author Spotlight: Combining Proximity Ligand Assay with Gamma-H2AX Staining to Characterize Protein Interactions in DNA Damage Response

Published on: August 2, 2024

898

Oleuropein Promotes DNA Damage in Prostate Cancer Cells via HIF-1α Downregulation

Hanping Wei1,2, Haoran Wu1,2, Wei Feng1,2

  • 1Department of Urology, Wujin Hospital Affiliated With Jiangsu University, Changzhou, China.

Journal of Biochemical and Molecular Toxicology
|September 30, 2025
PubMed

Insights

Oleuropein (Ole) inhibits prostate cancer cell viability and promotes apoptosis by inducing DNA damage. This effect is potentially mediated through the Hypoxia-Inducible Factor-1α (HIF-1α) pathway, suggesting Ole

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Prostate cancer (PCa) remains a significant global health concern.
  • Identifying novel therapeutic agents and understanding their mechanisms is crucial for effective PCa treatment.
  • Oleuropein (Ole), a natural compound, has shown potential anticancer properties.

Purpose of the Study:

  • To investigate the effects of Ole on PCa cells (DU145).
  • To elucidate the underlying mechanisms, focusing on DNA damage and the role of Hypoxia-Inducible Factor-1α (HIF-1α).
  • To evaluate Ole's potential as a therapeutic agent for PCa.

Main Methods:

  • Cell viability assessed using MTT assay.
  • Apoptosis detected by flow cytometry.
  • HIF-1α expression quantified via RT-qPCR and Western blot.
  • DNA damage evaluated using comet assay and Western blot for γH2AX and p53.

Main Results:

  • Ole significantly inhibited DU145 cell viability in a dose-dependent manner.
  • Ole treatment led to a significant increase in apoptosis rates.
  • Ole induced DNA damage in PCa cells, evidenced by comet assay and elevated γH2AX/p53 levels.
  • Overexpression of HIF-1α partially reversed Ole's inhibitory effects on PCa cells.

Conclusions:

  • Ole exhibits significant anticancer effects against PCa cells by inhibiting viability, promoting apoptosis, and inducing DNA damage.
  • The Hypoxia-Inducible Factor-1α (HIF-1α) pathway is implicated in mediating Ole's anticancer actions.
  • Ole shows promise as a potential therapeutic agent for prostate cancer, warranting further investigation.
Keywords:
DNA damagehypoxia‐inducible factor‐1αoleuropeinprostate cancer

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