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Related Experiment Video

Updated: Jan 16, 2026

RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells

RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells

Published on: February 13, 2013

Transcriptome Analysis Identified SPP1-Positive Monocytes as a Key in Extracellular Matrix Formation in Thrombi.

Takaya Kitano^1,2, Tsutomu Sasaki², Takahiro Matsui^3,4

¹Department of Neurology Toyonaka Municipal Hospital Osaka Japan.

Journal of the American Heart Association

|September 30, 2025

Summary

This summary is machine-generated.

Monocytes and macrophages expressing high levels of SPP1 are crucial for thrombus maturation. Targeting these cells may offer new strategies for treating thrombosis and preventing embolisms.

Keywords:

RNA sequencing extracellular matrix osteopontin stroke thrombosis

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Published on: November 23, 2017

Area of Science:

Biochemistry
Molecular Biology
Hematology

Background:

Thrombus stability influences natural course, persistence, and spontaneous dissolution.
Understanding thrombus maturation mechanisms is key for treating thrombosis and preventing embolisms.
Current knowledge on thrombus maturation processes remains incomplete.

Purpose of the Study:

To investigate the molecular mechanisms underlying thrombus maturation.
To identify key genes and cellular players involved in thrombus stability.
To explore potential therapeutic targets for thrombosis.

Main Methods:

RNA sequencing of cerebral thrombi and matched blood samples.
Immunohistochemistry validation on 66 cerebral embolism thrombi.
Single-cell RNA sequencing of pulmonary artery thrombi.

Main Results:

1121 genes were upregulated in thrombi, with extracellular matrix formation identified as a key pathway.
SPP1 (osteopontin) was identified as a hub gene, upregulated in thrombi.
SPP1-high monocytes/macrophages were identified, expressing extracellular matrix genes and correlating with thrombus resistance to thrombectomy.

Conclusions:

SPP1-high monocytes/macrophages play a critical role in thrombus maturation.
Osteopontin expression correlates with collagen type VI and thrombus resistance.
Targeting SPP1-expressing cells may be a novel therapeutic strategy for thrombosis.