Trace Detection of Staphylococcal Enterotoxin B Based on Hydrogen-Terminated Diamond Solution-Gate Field-Effect...

Area of Science:

• Nanotechnology
• Biosensors
• Analytical Chemistry

Background:

• Staphylococcal enterotoxin B (SEB) is a highly hazardous toxin produced by Staphylococcus aureus.
• Accurate and sensitive detection of SEB is crucial for public health, enabling timely prevention, diagnosis, and treatment.

Purpose of the Study:

• To develop a novel electrochemical platform for the sensitive and reliable trace detection of SEB.
• To utilize a hydrogen-terminated diamond solution-gate field-effect transistor (FET) for SEB sensing.

Main Methods:

• Fabrication of an electrochemical sensor using a diamond FET.
• Functionalization of the sensing area with 1-pyrenebutyric acid-N-hydroxysuccinimide ester and anti-SEB antibodies.
• Characterization of the sensor's surface using atomic force microscopy (AFM), scanning electron microscopy (SEM), and X-ray photoelectron spectroscopy (XPS).
• Evaluation of the device's output and transfer characteristics.

Main Results:

• Confirmation of successful antibody immobilization on the sensor surface.
• Demonstration of a wide linear response range for SEB detection, from 10^-15 to 10^-7 g/mL.
• Achieved high sensitivity of -49.37 mV/lg [SEB concentration].
• Established a remarkably low detection limit of 10^-15 g/mL.

Conclusions:

• The developed diamond FET-based electrochemical sensor provides a highly sensitive and reliable method for SEB trace detection.
• This platform holds significant potential for rapid and accurate SEB monitoring in various applications, contributing to enhanced safety and diagnostics.