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Trace Detection of Staphylococcal Enterotoxin B Based on Hydrogen-Terminated Diamond Solution-Gate Field-Effect
Yuxiang Du1, Qingyu Lv2, Yongqiang Jiang2
1Key Laboratory for Physical Electronics and Devices of the Ministry of Education & School of Science & Shaanxi Key Laboratory of Information Photonic Technique & Institute of Wide Bandgap Semiconductors, Xi'an Jiaotong University, Xi'an 710049, China.
None:
Staphylococcal enterotoxin B is the most hazardous of the various toxins produced by Staphylococcus aureus. Thus, trace detection of SEB is essential for prevention, diagnosis, and treatment. In this work, an electrochemical platform was constructed to detect SEB based on a hydrogen-terminated diamond solution-gate field-effect transistor. The connection of 1-pyrenebutyric acid-N-hydroxy succinimide ester and anti-SEB antibody on the sensing area was confirmed by atomic force microscopy, scanning electron microscopy, and X-ray photoelectron spectroscopy. Output and transfer characteristics of the device were investigated, which obtained a wide linear response from 10-15 to 10-7 g/mL, a high sensitivity of -49.37 mV/lg [SEB concentration], and a low detection limitation of 10-15 g/mL.
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