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Tandem Mass Spectrometry

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Related Experiment Video

Updated: Jan 16, 2026

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

Single molecule spectrum dynamics imaging with 3D target-locking tracking.

Hao Sha^1,2, Yu Wu^2,3, Yongbing Zhang^4,5

¹School of Computer Science and Technology, Harbin Institute of Technology (Shenzhen), Shenzhen, China.

Nature Communications

|September 30, 2025

Summary

This summary is machine-generated.

We developed a new microscopy technique, 3D-SpecDIM, to simultaneously track biomolecule movement and spectral changes in real-time. This breakthrough enables deeper understanding of dynamic biological processes at the single-molecule level.

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Area of Science:

Biophysics
Advanced Microscopy
Molecular Imaging

Background:

Fluorescence spectra provide detailed physicochemical information about molecular environments.
Simultaneously imaging rapid 3D positional and spectral dynamics of single biomolecules is challenging.

Purpose of the Study:

To introduce a novel microscopy method, 3D-SpecDIM, for simultaneous 3D positional and spectral dynamics imaging of single biomolecules.
To demonstrate the capability of 3D-SpecDIM in real-time biological process imaging.

Main Methods:

Developed three-dimensional target-locking-based single-molecule fluorescence Spectrum Dynamics Imaging Microscopy (3D-SpecDIM).
Achieved high spectral accuracy, fast spectral acquisition, single-molecule sensitivity, and high 3D spatiotemporal localization precision.
Applied 3D-SpecDIM to image mitophagy and cellular blebbing processes.

Main Results:

3D-SpecDIM successfully captured rapid 3D positional and physicochemical parameter dynamics simultaneously.
Demonstrated enhanced ratiometric fluorescence imaging during real-time mitophagy imaging.
Showcased multi-resolution imaging and quantitative imaging capabilities for cellular blebbing.

Conclusions:

3D-SpecDIM is a versatile platform for concurrent multiparameter dynamics acquisition.
Offers comprehensive insights into biomolecular environments and interactions unattainable with conventional methods.
Represents a significant advancement in single-molecule spectral dynamics imaging techniques.