Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

11.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.8K
Next-generation Sequencing03:00

Next-generation Sequencing

97.8K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
97.8K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

12.6K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
12.6K
DNA Packaging00:58

DNA Packaging

112.0K
Overview
112.0K
Genome Copying Errors02:46

Genome Copying Errors

5.0K
DNA replication is a well-evolved process that copies millions of base pairs with high fidelity during each cell division. Occasionally a wrong base or a long stretch of wrong bases may get added to the daughter strands. If the errors are left unchecked, cells might accumulate several mutations that might endanger their  survival. Therefore, the copying errors are checked and repaired at three levels.
5.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Female-Biased VSMC GRNs Predict MYH9 as Regulator of Fibrous Plaque Phenotype.

Circulation research·2026
Same author

Deficiency of <i>ZC3HC1</i> Modulates Vascular Smooth Muscle Cell Phenotype and Increases Neointima Formation.

Arteriosclerosis, thrombosis, and vascular biology·2026
Same author

A prevalent disease-associated SNP in the human <i>ID3</i> gene regulates E-protein activity and cellular proliferation.

NAR molecular medicine·2026
Same author

Atherosclerotic Fibrous Plaques in Women Present ECM Remodeling Linked to TGF-β.

Circulation research·2026
Same author

Adipocyte-specific ablation of plakoglobin in mice does not affect adiposity but results in sexual-dimorphic effects on weight gain.

Physiological reports·2025
Same author

Fibroblast mechanoperception instructs pulmonary developmental and pattern specification gene expression programs.

PLoS genetics·2025
Same journal

Correction: A method for supervoxel-wise association studies of age and other non-imaging variables from coronary computed tomography angiograms.

Scientific reports·2026
Same journal

Poly(bromophenol blue)/CoSn(OH)<sub>6</sub> cubic particles modified pencil graphite electrode for electrochemical determination of diphenhydramine.

Scientific reports·2026
Same journal

Dietary Chlorella, Spirulina, and acidifier modulate jejunal cytokine-related gene expression in broiler chickens.

Scientific reports·2026
Same journal

Perceived physical activity barriers in university students: associations with fatigue and eating behaviours.

Scientific reports·2026
Same journal

Refuge limitation structures habitat use in agricultural landscapes: evidence from Sunda pangolins.

Scientific reports·2026
Same journal

Lightweight stateless transaction verification with outsourced witness updates for UTXO blockchains.

Scientific reports·2026
See all related articles

Related Experiment Video

Updated: Jan 16, 2026

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

14.6K

Constrained coding for error mitigation in nanopore-based DNA data storage.

Kallie Whritenour1, Mete Civelek2, Farzad Farnoud3

  • 1Computer Science, University of Virginia, Charlottesville, USA.

Scientific Reports
|September 30, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces a novel coding system to enhance DNA data storage reliability. By reducing nanopore sequencing errors up to sixfold, it significantly improves data integrity for DNA-based digital information.

More Related Videos

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores
09:43

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

Published on: October 31, 2013

14.1K
Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example
05:45

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Published on: March 11, 2020

9.3K

Related Experiment Videos

Last Updated: Jan 16, 2026

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

14.6K
Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores
09:43

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

Published on: October 31, 2013

14.1K
Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example
05:45

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Published on: March 11, 2020

9.3K

Area of Science:

  • Biotechnology
  • Bioinformatics
  • Data Storage

Background:

  • DNA data storage offers a high-density alternative to traditional digital storage.
  • Nanopore sequencing enables real-time DNA data retrieval but suffers from lower accuracy.
  • High error rates in nanopore sequencing pose a challenge for reliable DNA data storage.

Purpose of the Study:

  • To develop a constrained coding system to mitigate nanopore sequencing errors.
  • To improve the reliability and accuracy of data retrieval in DNA data storage systems.

Main Methods:

  • Designed constrained codes utilizing de Bruijn graphs.
  • Implemented a state-splitting encoder and a Viterbi-based decoder.
  • Simulated data encoding and decoding, including image recovery.

Main Results:

  • The novel coding system significantly reduces sequence-level errors compared to existing methods.
  • Achieved up to a sixfold reduction in errors, enhancing data integrity.
  • Successfully demonstrated image recovery, validating the system's effectiveness.

Conclusions:

  • Constrained coding is a viable strategy to overcome nanopore sequencing limitations for DNA data storage.
  • The developed system offers a substantial improvement in reliability for DNA-based digital data storage.
  • This approach paves the way for more robust and practical DNA data storage solutions.