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Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment
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Profiling of Macrophage Polarization Using Automated Enzyme-Linked Immunosorbent Assay
Simon Loevenich1, Vu To Nakstad1, Hanne Hein Trøen1
1Department of Biotechnology and Nanomedicine, SINTEF Industry, Trondheim, Norway.
Methods in Molecular Biology (Clifton, N.J.)
|September 30, 2025
Abstract
None:
Macrophage activation has emerged as a key area in immunology, in which they are polarized to adopt different functional phenotypes in response to various environmental signals. Here, we describe the procedure of isolating human primary CD14+ monocytes and polarization toward M1 (pro-inflammatory) and M2 (anti-inflammatory) phenotypes. Relevant cytokine levels can be detected by manual and automated enzyme-linked immunosorbent assays (ELISA).
