Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Mouse Models of Cancer Study

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Transferrin in combination with induction chemotherapy improves outcomes in mouse models of acute myeloid leukemia.

Science translational medicine·2026

Same author

Systemic and Local Adiposity in the Bone Marrow Microenvironment Associated With Improved Prognosis in Hodgkin Lymphoma: Imaging and Molecular Analysis.

International journal of cancer·2026

Same author

Radiotherapy induces layer-specific molecular and ultrastructural alterations in triple-negative breast cancer spheroids.

Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology·2026

Same author

Novel rectal cancer spheroid models reveal metabolic and transcriptional adaptations driving radiotherapy response.

Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology·2025

Same author

Author Correction: Sequential orthogonal assays for longitudinal and endpoint characterization of three-dimensional spheroids.

Nature protocols·2025

Same author

Sequential orthogonal assays for longitudinal and endpoint characterization of three-dimensional spheroids.

Nature protocols·2025

Search research articles

Related Experiment Video

Updated: Jul 29, 2026

A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds

A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds

Published on: April 6, 2016

Cancer Patient-Derived Scaffolds as In Vitro Preclinical Models for Immunotherapy Screening.

Diogo Estêvão^1,2, Maria J Oliveira^3,4, Tânia B Cruz¹

¹i3S-Institute for Research and Innovation in Health, University of Porto, Porto, Portugal.

Methods in Molecular Biology (Clifton, N.J.)

|September 30, 2025

Summary

Patient-derived decellularized extracellular matrix (ECM) offers advanced 3D biomimetic models for cancer research. These models accurately mimic tumors, aiding in high-throughput screening for cancer therapies, especially immunotherapies.

Keywords:

Biomimetic models Decellularization Extracellular matrix Patient-derived scaffolds Therapy screenings

More Related Videos

Quantifying Antibody-Dependent Cellular Cytotoxicity in a Tumor Spheroid Model: Application for Drug Discovery

Quantifying Antibody-Dependent Cellular Cytotoxicity in a Tumor Spheroid Model: Application for Drug Discovery

Published on: April 26, 2024

Heteromulticellular Stromal Cells in Scaffold-free 3D Cultures of Epithelial Cancer Cells to Drive Invasion

Heteromulticellular Stromal Cells in Scaffold-free 3D Cultures of Epithelial Cancer Cells to Drive Invasion

Published on: April 4, 2025

Related Experiment Videos

Last Updated: Jul 29, 2026

A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds

A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds

Published on: April 6, 2016

Quantifying Antibody-Dependent Cellular Cytotoxicity in a Tumor Spheroid Model: Application for Drug Discovery

Quantifying Antibody-Dependent Cellular Cytotoxicity in a Tumor Spheroid Model: Application for Drug Discovery

Published on: April 26, 2024

Heteromulticellular Stromal Cells in Scaffold-free 3D Cultures of Epithelial Cancer Cells to Drive Invasion

Heteromulticellular Stromal Cells in Scaffold-free 3D Cultures of Epithelial Cancer Cells to Drive Invasion

Published on: April 4, 2025

Area of Science:

Biomedical Engineering
Cancer Biology
Extracellular Matrix Research

Background:

The extracellular matrix (ECM) is crucial for tissue structure and signaling within the tumor microenvironment (TME).
ECM significantly impacts cancer progression, including proliferation, invasion, and metastasis.
Accurate in vitro cancer models require the inclusion of ECM components.

Purpose of the Study:

To present innovative protocols for creating 3D biomimetic cancer models.
To highlight the utility of patient-derived decellularized ECM in these models.
To establish these models as high-throughput strategies for cancer research and immunotherapy screening.

Main Methods:

Exploration of various scaffold materials (natural/synthetic polymers, ceramics).
Focus on decellularized ECM derived from patient tumors.
Development of advanced protocols for obtaining 3D decellularized ECM models.

Main Results:

Decellularized ECM preserves native architecture, biomechanics, and molecular composition.
These models offer a distinct advantage over other biomimetic approaches.
The developed protocols enable high-throughput strategies.

Conclusions:

Patient-derived decellularized ECM provides superior biomimetic 3D cancer models.
These models are valuable tools for advancing cancer research.
The approach is particularly promising for immunotherapy screening.