Comprehensive Method for Soybean Cultivar Identification Using MGIseq, VCF2GENOME, and REST API

This protocol provides a comprehensive method for identifying soybean (Glycine max) cultivars using a combination of advanced genomic and bioinformatic techniques. The process begins with DNA extraction from soybean leaf tissue, followed by MGIseq library construction and high-throughput sequencing. The resulting data undergoes rigorous quality control and preprocessing before being mapped to the soybean reference genome. Variant calling is then performed to generate a Variant Call Format (VCF) file, which is subsequently analyzed using the web application, VCF2GENOME and the REST API. The API facilitates the comparison of the sample's genetic profile with a curated database of known soybean cultivars, enabling accurate identification. This method is designed for researchers, breeders, and seed companies who require high-throughput, high-accuracy cultivar identification for various applications in soybean genetics, breeding, and seed quality control.