Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

1.7K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.7K
CRISPR01:59

CRISPR

57.5K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
57.5K
CRISPR and crRNAs02:53

CRISPR and crRNAs

18.7K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
18.7K
Homologous Recombination02:31

Homologous Recombination

62.6K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
62.6K
What is Genetic Engineering?00:49

What is Genetic Engineering?

79.6K
Overview
79.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrative and interpretable machine learning framework for early non-invasive detection of clinically significant liver fibrosis.

Frontiers in medicine·2026
Same author

Design of novel interlocked bi-layer NiTi braided stent with ultra-thin walls for urinary tract obstruction treatment.

Regenerative biomaterials·2026
Same author

Construction of a high-quality indexed EMS mutant library and a pipeline for identifying causal mutations in wheat by whole-exome sequencing.

Journal of genetics and genomics = Yi chuan xue bao·2026
Same author

Astrocytic FTO-dependent m6A demethylation drives sevoflurane-induced perioperative neurocognitive disorders in mice.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2026
Same author

Interaction between Per- and Polyfluoroalkyl Substances and Dissolved Organic Matter in Soil: Molecular Variation and Partitioning Behavior.

Environmental science & technology·2026
Same author

Short-term development of neurocognition in early-stage schizophrenia with predominant negative symptoms.

Psychiatry research·2026

Related Experiment Video

Updated: Jan 16, 2026

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

3.3K

CRISPR/Cas9-Based Gene Editing in Soybean.

Hongli Yang1, Aili Bao1, Lam-Son Phan Tran2

  • 1Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, China.

Methods in Molecular Biology (Clifton, N.J.)
|October 1, 2025
PubMed
Summary
This summary is machine-generated.

This study details a robust CRISPR/Cas9 gene editing method for soybean. It provides protocols for plasmid construction and Agrobacterium-mediated transformation, enabling efficient soybean mutant generation.

Keywords:
Agrobacterium-mediated soybean transformationCRISPR/cas9Cotyledonary nodeGene editingSoybeanTissue culture

More Related Videos

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
09:22

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

Published on: January 25, 2018

26.2K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

35.5K

Related Experiment Videos

Last Updated: Jan 16, 2026

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

3.3K
A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
09:22

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

Published on: January 25, 2018

26.2K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

35.5K

Area of Science:

  • Agricultural Science
  • Molecular Biology
  • Genetics

Background:

  • CRISPR/Cas9 is a powerful tool for crop functional genomics and breeding.
  • Soybean gene editing has been achieved, but detailed methods are lacking.

Purpose of the Study:

  • To establish a detailed CRISPR/Cas9 gene editing protocol for soybean.
  • To enable efficient generation of soybean mutants.

Main Methods:

  • Construction of a CRISPR/Cas9 plasmid for soybean.
  • Modified Agrobacterium-mediated soybean transformation.
  • Regeneration of transgenic soybean from cotyledonary node explants.

Main Results:

  • A detailed CRISPR/Cas9 plasmid construction method was developed.
  • Optimized protocols for soybean transformation and regeneration were established.
  • The method facilitates the creation of soybean mutants.

Conclusions:

  • This work provides a comprehensive protocol for CRISPR/Cas9 gene editing in soybean.
  • The described methods will advance soybean functional genomics and breeding programs.