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Updated: Jan 16, 2026

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Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing
Published on: March 31, 2017
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Isolation of Pichia pastoris Genomic DNA for Long-Read DNA Sequencing and PCR Applications
Sven Heimhilcher1, David Keßler2, Lukas Rieder3
1bisy GmbH, Wünschendorf, Hofstätten a.d. Raab, Austria.
Methods in Molecular Biology (Clifton, N.J.)
|October 1, 2025
Abstract:
Nanopore sequencing applications allow for the rapid acquisition of genetic information. However, the fast and cost-efficient extraction of high molecular-mass genomic DNA from Komagataella phaffii can become a bottleneck in setting up an effective sequencing pipeline. In this chapter, we describe two methods for the extraction of genomic DNA that are suitable for downstream PCR applications and for long-read genomic DNA sequencing via a Nanopore sequencing device, respectively.

