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Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
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Adaptive Laboratory Evolution of Pichia pastoris by Serial Batch Cultivation

Charles Moritz1,2, Thomas Gassler3, Martin Altvater4

  • 1Department of Biotechnology, Institute of Microbiology and Microbial Biotechnology, BOKU University, Vienna, Austria. charles.moritz@boku.ac.at.

Methods in Molecular Biology (Clifton, N.J.)
|October 1, 2025
PubMed
Abstract

Keywords:
Adaptive laboratory evolutionKomagataella phaffiiPichia pastorisReverse engineeringSerial batch cultivationStrain engineering

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