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Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
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Protein Purification Protocols for Recombinant Enzymes Produced in Pichia pastoris

Merve Keser1, Mikel Dolz2, Javier Viña-Gonzalez2

  • 1Department of Biocatalysis, Institute of Catalysis, CSIC, Madrid, Spain.

Methods in Molecular Biology (Clifton, N.J.)
|October 1, 2025
PubMed

Keywords:
BioreactorEnzyme heterologous expressionImmobilized metal affinity chromatography (IMAC)Ion exchange chromatography (IEC)Komagataella phaffiiPichia pastorisProtein purification

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