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Updated: Jan 16, 2026
VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance
Published on: August 23, 2013
A Novel Approach Using SSR Markers for Identifying and Breeding Bruchid Pest-Resistant Blackgram (Vigna mungo L.
Ragul Subramaniyan1, Manivannan Narayana2, Soundararajan Raga Palanisamy3
1Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, India.
Abstract:
Molecular and biochemical techniques are beneficial in generating markers to investigate interesting genes in populations. SSRs, as genetic markers, provide significant advantages over traditional techniques of breeding and identifying essential traits. Under storage circumstances, the bruchid beetle Callosobruchus sp. can cause significant yield loss in blackgram. Hence, the identification of bruchid-resistant lines and breeding for resistance are important in blackgram. In this paper, the screening technique useful for the bruchid resistance breeding program and the use of SSR markers in identifying resistance are presented.
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01:20Monohybrid Crosses
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Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...