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Related Experiment Video

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Methods to Isolate Enteric Glia from hPSC-Derived Enteric Ganglioids.

Evgeniia Pimenova1, Faranak Fattahi2,3,4, Homa Majd5,6

  • 1Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 1, 2025
PubMed
Summary

Researchers developed a method to generate human enteric glia from stem cells. These cells mimic natural enteric glia, offering a new model for studying gut nervous system diseases and therapies.

Keywords:
DGBIDrug screeningEnteric neuronsGliosisGutIn vitro differentiationPeripheral glia

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Area of Science:

  • Neuroscience
  • Stem Cell Biology
  • Gastroenterology

Background:

  • Enteric glia are crucial for enteric nervous system (ENS) homeostasis and function.
  • Studying human enteric glia is challenging due to difficulties in primary tissue isolation.
  • Understanding enteric glial diversity and roles in neuropathies requires accessible cell models.

Purpose of the Study:

  • To establish a reliable method for generating enteric glia from human pluripotent stem cells (hPSCs).
  • To characterize hPSC-derived enteric glia for their similarity to primary human enteric glia.
  • To provide a scalable platform for studying enteric glial biology and disease.

Main Methods:

  • Differentiated hPSCs into enteric glia via vagal and enteric neural crest intermediates.
  • Confirmed cell identity using canonical marker expression (SOX10, GFAP, PLP1, S100B).
  • Utilized single-nucleus RNA sequencing (snRNA-seq) to assess cellular diversity.

Main Results:

  • Successfully generated scalable cultures of enteric glia from hPSCs.
  • hPSC-derived enteric glia expressed key markers and exhibited cellular diversity comparable to primary cells.
  • These cultures support studying glial interactions with immune cells, epithelial cells, and the microbiome.

Conclusions:

  • hPSC-derived enteric glia provide a robust and reproducible model for enteric neurobiology research.
  • This platform facilitates disease modeling and therapeutic discovery for enteric neuropathies.
  • Advancement in understanding enteric glial cell functions in health and disease is enabled.