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Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.

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Related Experiment Video

Updated: Jan 16, 2026

In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters

In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters

Published on: July 4, 2018

Splice Modulation Studies Using Reporter Mice.

Katsura Minegishi¹, Tsukasa Tominari¹, Yoshitsugu Aoki²

¹Department of Molecular Therapy, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo, Japan.

Methods in Molecular Biology (Clifton, N.J.)

|October 1, 2025

Summary

This summary is machine-generated.

Antisense oligonucleotides (ASOs) offer exon-skipping therapy for Duchenne muscular dystrophy (DMD). A novel reporter mouse model enables noninvasive, high-throughput evaluation of ASO efficacy for DMD treatment.

Keywords:

Duchenne muscular dystrophy Exon skipping Fluorescence In vivo imaging system (IVIS)Luciferase Reporter mouse

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Last Updated: Jan 16, 2026

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Published on: July 4, 2018

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A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Area of Science:

Biochemistry
Genetics
Molecular Biology

Background:

Duchenne muscular dystrophy (DMD) is a genetic disorder characterized by progressive muscle degeneration.
Exon-skipping therapy using antisense oligonucleotides (ASOs) aims to restore dystrophin protein production by correcting the DMD gene's reading frame.
Current methods for evaluating ASO efficacy, such as RT-PCR and immunoblotting on biopsied tissues, are labor-intensive and low-throughput.

Purpose of the Study:

To develop a novel, high-throughput, and noninvasive method for evaluating the efficacy of exon-skipping ASOs in vivo.
To demonstrate the utility of reporter mice for monitoring ASO-induced exon skipping and subsequent protein expression.

Main Methods:

Development of reporter mice engineered to express fluorescent proteins upon successful exon skipping.
Administration of antisense oligonucleotides (ASOs) designed to induce exon skipping in the dystrophin gene.
Noninvasive monitoring of fluorescent protein expression in reporter mice to assess therapeutic efficacy.

Main Results:

Exon skipping induced by ASOs resulted in the expression of fluorescent proteins in reporter mice.
The reporter system allowed for real-time, noninvasive assessment of ASO-mediated exon skipping.
This in vivo approach provides a high-throughput platform for evaluating next-generation ASO therapies.

Conclusions:

Reporter mice offer a powerful tool for the high-throughput, noninvasive evaluation of exon-skipping ASO efficacy for Duchenne muscular dystrophy.
This novel method streamlines the assessment of ASO-based therapeutic strategies, accelerating drug development.
The in vivo reporter system facilitates efficient screening of ASO sequences and modifications for optimal exon-skipping induction.