Development of Electric Field Stimulation (EFS)-Mediated Skeletal Muscle Training with iPSCs

Functional analysis of skeletal muscle in vitro requires maturation and stimuli to induce excitation-contraction coupling as well as a quantitative method to analyze contraction. Electric field stimulation (EFS) has been traditionally used as a method to excite skeletal muscle in vitro and in vivo. A combination of human-induced pluripotent stem cell (hiPS) technology and EFS will lead to recapitulation of functional phenotypes of skeletal muscle diseases. Here, we describe the combinational technology using hiPS technology and EFS to induce the maturation of skeletal muscle and analyze contractile performance in vitro.