Mapping of the Ethylene-Specific Root System Architecture (RSA) Traits in Arabidopsis

Understanding the root system architecture (RSA) is necessary for elucidation of plant growth patterns in response to environmental stimuli and hormonal signals. Ethylene, a gaseous phytohormone, modulates root developmental plasticity, including primary root elongation, lateral root formation, and root hair growth. We present a protocol for mapping ethylene-specific RSA traits in Arabidopsis thaliana using a hydroponic growth system. Arabidopsis seedlings grow on a polypropylene mesh supported by polycarbonate wedges in a magenta box-based setup. We treat seedlings with ethylene or its precursor, then spread root system on agar plates with an art brush. High-resolution images are recorded and analyzed with free ImageJ software. This protocol allows detailed RSA analysis under controlled ethylene treatments and can be adapted for other plant species.