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Related Experiment Video

Updated: Jan 16, 2026

Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy

Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy

Published on: December 3, 2012

Nano-org, a functional resource for single-molecule localisation microscopy data.

S Shirgill¹, D J Nieves¹, J A Pike^2,3,4

¹School of Infection, Inflammation and Immunology, School of Mathematics, Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham, Birmingham, UK.

Nature Communications

|October 1, 2025

Summary

This summary is machine-generated.

We developed nano-org, a searchable database of single-molecule localisation microscopy (SMLM) data. This resource aids in comparing protein nanoscale organization across experiments, advancing spatial nano-omics research.

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Area of Science:

Cellular Biology
Biophysics
Microscopy

Background:

Protein nanoscale organization is crucial for cellular functions like signaling and adhesion.
Single-molecule localisation microscopy (SMLM) provides high-resolution spatial data on protein distribution.
Comparing SMLM data across studies is difficult due to limited functional resources.

Purpose of the Study:

To create a publicly accessible, curated resource for SMLM data.
To enable comparative analysis of protein nanoscale organization.
To support the emerging field of spatial nano-omics.

Main Methods:

Development of the 'nano-org' database.
Inclusion of SMLM datasets detailing protein nanoscale distributions.
Implementation of a search function based on statistical similarity of datasets.

Main Results:

A publicly available, searchable resource named nano-org has been established.
The resource contains curated SMLM data on protein nanoscale organization.
Search functionality allows comparison of datasets based on statistical similarity.

Conclusions:

Nano-org facilitates the comparison of protein nanoscale architectures across different cellular contexts.
This resource supports research into protein organization and spatial nano-omics.
It addresses the challenge of data comparability in SMLM studies.