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Spatiotemporal regulation of endoplasmic reticulum stress visualized by live imaging of bipA mRNA in Aspergillus

Pakornswit Sathongdejwisit1, Yuki Morita1, Yoshinori Katakura1

  • 1Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 744 Motooka, Fukuoka, 819-0395, Japan.

Scientific Reports
|October 1, 2025
PubMed

Insights

This study investigates the endoplasmic reticulum (ER) stress response in Aspergillus oryzae, revealing how bipA mRNA dynamics are regulated during ER stress. We observed bipA mRNA expression and movement, providing insights into fungal cellular biology.

Area of Science:

  • Cellular Biology
  • Molecular Biology
  • Mycology

Background:

  • Endoplasmic reticulum (ER) stress response mechanisms are well-understood in many eukaryotes.
  • Limited cellular biological knowledge exists regarding ER stress response in filamentous fungi.
  • Aspergillus oryzae is a filamentous fungus with industrial and pathogenic relevance.

Purpose of the Study:

  • To elucidate the cellular mechanisms of ER stress response in Aspergillus oryzae.
  • To analyze the spatiotemporal dynamics of bipA mRNA during ER stress.
  • To investigate the regulation of intracellular mRNA levels in response to ER stress.

Main Methods:

  • Utilized the MS2 system for live-cell imaging of bipA mRNA in Aspergillus oryzae.
  • Employed fluorescence microscopy to observe temporal and spatial changes in bipA mRNA.
  • Applied dithiothreitol (DTT) to induce ER stress and cycloheximide to inhibit translation.

Main Results:

  • Observed temporal changes in bipA mRNA levels in response to DTT-induced ER stress.
  • Found bipA mRNA expression throughout hyphal cells, from apical to basal regions.
  • Demonstrated that while some bipA mRNA exhibits microtubule-dependent dynamics, most is static, suggesting localized translation.

Conclusions:

  • Clarified the gene expression mechanism of bipA in response to ER stress in A. oryzae.
  • Provided insights into the regulation of intracellular mRNA levels and dynamics during fungal ER stress.
  • Highlighted the role of mRNA localization and dynamics in the cellular response to ER stress in filamentous fungi.
Keywords:
Aspergillus oryzaeEndoplasmic reticulum stressFilamentous fungiMicrotubulemRNA

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