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Related Concept Videos

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Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
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During ejaculation, males release around 2-5 milliliters of semen, which is a complex mixture of mature sperm and various fluids produced by accessory glands. The mature sperm cells measure approximately 60 micrometers in length and consist of a head, neck, midpiece, and tail. The head is flattened and tapered, measuring about 4 to 5 micrometers in length. It contains a nucleus with condensed chromosomes and an acrosome, a cap-like structure filled with enzymes essential for penetrating the...
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Selenium-GPX4 Axis Relieves Arachidonic Acid-Induced Sperm Damage.

Huihui Tian1, Jiaying Chen1, Shijie Fan2

  • 1State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Beijing 100193, China.

Journal of Agricultural and Food Chemistry
|October 1, 2025
PubMed
Summary
This summary is machine-generated.

Selenium and glutathione peroxidase 4 protect male fertility from damage caused by excessive arachidonic acid. This axis regulates metabolites, reduces oxidative stress, and improves sperm quality.

Keywords:
arachidonic acidglutathione peroxidase 4seleniumsperm damage

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Area of Science:

  • Reproductive Biology
  • Nutritional Biochemistry
  • Toxicology

Background:

  • Selenium (Se) and glutathione peroxidase 4 (GPX4) are critical for male reproductive health, involved in spermatogenesis and protection against oxidative damage.
  • Arachidonic acid (AA) impacts male fertility, with excessive intake leading to adverse effects on sperm quality.
  • The precise mechanisms by which the Se-GPX4 axis mitigates AA-induced testicular and sperm injury are not fully understood.

Purpose of the Study:

  • To investigate how the Selenium-GPX4 axis ameliorates arachidonic acid-induced testicular and sperm injury.
  • To elucidate the underlying molecular mechanisms, including omics-based approaches.

Main Methods:

  • Establishment of an arachidonic acid-induced sperm injury model in vivo.
  • Administration of Selenium (Se) supplementation.
  • Utilisation of testicular-specific GPX4 knockout models.
  • Comprehensive analysis using oxylipidomics and transcriptomics.

Main Results:

  • Selenium supplementation significantly improved testicular and sperm quality in the AA-induced injury model by regulating GPX4 expression.
  • GPX4 knockout exacerbated AA-induced testicular and sperm damage.
  • Se-GPX4 axis intervention alleviated oxidative stress, apoptosis, sex hormone disorders, and inflammation.
  • Oxylipidomics identified alterations in testicular polyunsaturated fatty acid (PUFA) metabolites, while transcriptomics highlighted key affected pathways.

Conclusions:

  • The Se-GPX4 axis plays a crucial role in ameliorating AA-induced sperm damage.
  • This protective effect involves regulating PUFA metabolism, activating glutathione metabolism, and mitigating oxidative stress, apoptosis, and inflammation.
  • The axis also contributes to the repair of steroid hormone biosynthesis, thereby preserving male reproductive function.