Related Experiment Video
Updated: Jan 16, 2026
16:42
Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides
Published on: November 24, 2010
27.3K
Transposase Acting on an RNA/DNA Hybrid in Strand-Specific Sequencing
Shihui Zhao1,2, Yanyi Huang1,2,3, Chunhong Zheng1,4
1Biomedical Pioneering Innovation Center (BIOPIC), Peking University International Cancer Institute, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.
Analytical Chemistry
|October 1, 2025
Summary
Directional SHERRY (d-SHERRY) is a new RNA sequencing method that simplifies workflows and improves accuracy. This strand-specific technique enhances transcriptome profiling, even with minimal RNA input.
Area of Science:
- Molecular Biology
- Genomics
- Transcriptomics
Background:
- Strand-specific RNA sequencing is vital for understanding gene regulation.
- Current methods face challenges balancing sensitivity, accuracy, and ease of use.
Purpose of the Study:
- To develop a streamlined, strand-specific RNA sequencing method.
- To improve sensitivity, accuracy, and workflow simplicity in transcriptome profiling.
Main Methods:
- Introduced directional SHERRY (d-SHERRY), a method using Tn5 transposase on RNA/DNA hybrids.
- Eliminated the need for second-strand cDNA synthesis.
- Optimized reverse transcription and tagmentation conditions.
Main Results:
- Achieved over 95% strand specificity.
- Detected over 10,000 genes from 100 pg of RNA.
- Demonstrated superior library complexity and coverage uniformity compared to commercial kits.
- Resolved complex genomic regions, including overlapping antisense transcripts, with >98% specificity.
Conclusions:
- d-SHERRY offers a rapid, sensitive, and reliable solution for strand-specific transcriptome profiling.
- The method is effective across diverse sample types and input amounts.
- Streamlined workflow with minimal hands-on time (0.5-1 h).
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