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Water-Soluble Squaraine-Based NIR Chromofluorogenic Probe for HClO/ClO- Detection in Environmental Waters and
Huifang Li1, Wanying Li1, Yiru Tang1
1Green Pharmaceutical Technology Key Laboratory of Luzhou City, Central Nervous System Product Research and Development Key Laboratory of Sichuan Province, School of Pharmacy, Southwest Medical University, Luzhou, 646000, PR China.
Abstract:
Hypochlorous acid/hypochlorite (HClO/ClO-), as critical biological reactive oxygen species (ROS), plays essential roles in numerous physiological and pathological processes. To enable sensitive detection of HClO/ClO- in environmental water samples and living cells, a near-infrared (NIR) water-soluble squaraine-based chromofluorogenic probe SO3SQ was developed. SO3SQ exhibits good water solubility, NIR detection wavelength (696 nm/725 nm), high selectivity, rapid response time (within seconds), visible colorimetric response, and minimal cytotoxicity. Furthermore, SO3SQ achieved quantitative HClO/ClO- detection in environmental water samples and realized real-time fluorescence imaging of exogenous/endogenous HClO/ClO- in HepG2 cells. This work provides new ideas for the future design of NIR water-soluble HClO/ClO- probes, while expanding the application of squaraine dyes as HClO/ClO- probes.
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