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Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Updated: Jan 16, 2026

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

Minimally Invasive DNA-Mediated Photostabilization for Extended Single-Molecule and Super-resolution Imaging.

Michael Scheckenbach¹, Cindy Close¹, Julian Bauer¹

¹Department of Chemistry and Center for NanoScience, Ludwig-Maximilians-Universität München, Butenandtstr. 5-13, 81377 München, Germany.

Biorxiv : the Preprint Server for Biology

|October 1, 2025

Summary

This summary is machine-generated.

We developed a DNA-mediated photostabilization method to prevent fluorescence photobleaching in microscopy. This minimally invasive technique improves imaging quality and expands fluorophore options for super-resolution microscopy.

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Area of Science:

Biophysics
Microscopy
Molecular Imaging

Background:

Photobleaching limits single-molecule and super-resolution microscopy.
Conventional photostabilization methods have drawbacks like fluorophore selection and biological disruption.

Purpose of the Study:

To develop a modular, minimally invasive photostabilization approach using DNA-mediated delivery.
To overcome limitations of conventional photostabilization methods in microscopy.

Main Methods:

Utilized DNA-mediated delivery of photostabilizers to the imaging site.
Implemented a recovery scheme for continuous photostabilizer replenishment.
Applied the method to 3D-DNA PAINT cell imaging.

Main Results:

DNA-mediated strategy outperformed solution-based methods at lower excitation intensities.
Achieved efficient photostabilization at significantly lower additive concentrations.
Demonstrated improved localization rate and precision in 3D-DNA PAINT cell imaging.

Conclusions:

DNA-mediated photostabilization is a promising solution for imaging where high additive concentrations are prohibited.
The modularity allows adaptation to various imaging schemes and expands suitable fluorophores.
This approach enhances capabilities in single-molecule and super-resolution imaging.